Preparation of Manuscripts – regular paper and minireview

The manuscript of the full-length original paper or minireview in general:

  • Should not exceed 30 typed pages (up to 1800 characters per page without spaces) including tables and figures, each on separate page
  • Be typed in 12 points Times New Roman font
  • Every portion of the manuscript should be double-spaced
  • No part of the manuscript should be underlined and written using capital letters

The full length paper should be divided into the following sections written in sequence: Title, Abstract, Introduction, Experimental: Materials and Methods, Results, Discussion, Acknowledgments, Literature.


The title should briefly describe the contents of the paper. Below the title, the manuscript should include full name (including first name and middle initial) of each Author and affiliations of all Authors. Street address, telephone number and e-mail address should be given only for the corresponding Author (or corresponding Authors, when apply) and placed in the footnote at the bottom of the first page. Under the Authors affiliations the proposed running title should be included (50 characters including spaces).


Limit the Abstract to 250 words or fewer. Because the abstract will be published separately by abstracting services, it must be complete and understandable without reference to the text. It should be written in an impersonal form. Abbreviations, diagrams and references are not allowed.


Five keywords or short phrases should be given below the abstract. If names of microorganisms are used, they should precede the key words, and be followed by the latter in alphabetical order. All keywords should be relevantly connected with the subject matter (avoid common terms like: bacteria, medium, soil, temperature etc.) as they will be used for indexing purposes.


The introduction should provide background information to allow the reader to understand and evaluate the results of the present study and describe the purpose of the undertaken research. However, broad “academic lectures” on the subject and extensive literature reviews should be avoided.


Materials & Methods

This section should contain description of materials (biological and others) used and sufficient technical information so that the experiments can be repeated. For commonly used materials and methods (e.g. commonly used media, protein determination) a simple reference is sufficient. Novel or modified procedures should be described in detail.
When a large number of microbial strains or mutants were used in a study, include strain tables identifying the sources and properties of the strains, mutants, bacteriophages, plasmids, etc.


In the Results section, only the results of the experiments should be included; reserve extensive interpretation of the results for the Discussion section. When justified by the nature of the paper the Results and Discussion sections may be combined into Results and Discussion chapter. The results should be presented as concisely as possible and illustrated with tables or figures if applicable (presentation of the same results in both tables and figures is not acceptable). All Tables and Figures must be referred to in the main body of the text.


The Discussion should provide an interpretation of the obtained results in relation to previously published work and to the experimental system at hand and should not contain extensive repetition of the Results section or reiteration of the introduction. In some papers the Results and Discussion can be combined into one section as mentioned above.


Acknowledgements for financial support and for a personal assistance (with the permission of person named) are given in two separate paragraphs below the main text.

Literature (and references)

In-text references and the reference list at the end of the paper should be prepared according to the Council of Science Editors (CSE) documentation Name-Year (N-Y) system.

In-text references should be cited in the form of the author’s last name and the year of publication, in parentheses, e.g. (Nowak 2017). When a paper has two authors, it should be cited in parentheses as follows: (Nowak and Smith 2017).

When a paper has more than two authors, the first author’s name should be followed with “et al.” and the year of publication, e.g. (Nowak et al. 2002). While references occur that are not identified by the authors’ names and year, use a, b etc. after the year (Nowak et al. 2002a; 2002b).

When you cite multiple works by different authors, put the sources in chronological order from oldest to most recent (and alphabetically, if published in the same year). Separate studies by semicolon: (Nowak et al. 2008; Smith et al. 2016).

The list of the papers cited (Literature) is organized alphabetically according to the last name of the first author and not numbered. The reference list is organized alphabetically by author’s last name. When there is more than one work by the author, those works are organized chronologically. Authors’ first names are rendered as capitals after their surnames as in an example: Nowak BS, Smith J, Coward PP. Papers with one only author are listed in chronological order (the earliest first); papers with two authors are listed alphabetically according to the last name of the first author and by the last name of the second author; paper with three or more authors appear as those with two authors and are listed chronologically.

Only the first word of a book or article title should be capitalized. Titles are not italicized. However, species names are italicized.

Here, you find an example: Nowak PP, Smith J. 2011. Occurrence of OxyR in the hydrogen peroxide stress response of Pseudomonas aeruginosa. J Clin Microbiol. 75(12):534-536.

The journal article on the Internet should be cited as follows:

Nowak NG, Smith TY, Howard FG. 2017. Archaea and bacteria. Biol Microbiol [Internet] [cited 2017 May 15]; 158(1):1-25. Available from: doi:17.1111/j.1744-7348.2010.y

References for books follow should be provided in the order: Author(s). Year. Title. Edition. Place of publication: Publisher. Extent.

As in an example: Nowak C, Smith P, Howard M. 2008. Virulence factors of Pseudomonas aeruginosa. Warsaw: PTM Press 203 p.

References for chapters or other parts of a book should be provided in the order: Author(s). Year. Chapter title. In: Editor(s). Book title. Place of publication: Publisher. Page numbers for that chapter.

Use the proper journal title abbreviations (ISO4 standard) as indicated in the PubMed Journal Database.

The Editorial Office strongly recommends to use the dedicated software to manage and edit citations and literature. To easily adjust or change references according to the PJM requirements choose a CSE citation style (N-Y option), which is build-in in these programs. List and comparison of reference management software may be viewed e.g. at the Wikipedia site.