Preparation of Manuscripts – regular paper and minireview

The manuscript of the full length original paper or minireview in general:

  • Should not exceed 30 typed pages (up to 1800 characters per page) including tables and figures
  • Be typed in 12 points Times New Roman font
  • Every portion of the manuscript should be double-spaced
  • No part of the manuscript should be underlined and written using capital letters.

The full length paper should be divided into the following sections written in sequence: Title, Abstract, Introduction, Experimental: Materials and Methods, Results, Discussion, Acknowledgments, Literature.


The title should briefly describe the contents of the paper. Below the title, the manuscript should include full name (including first name and middle initial) of each author and affiliations of all authors. Street address, telephone number and e-mail address should be given only for the corresponding author and placed in the footnote at the bottom of the first page.
Under the authors affiliations proposed running title should be included (50 characters including spaces).


Limit the abstract to 250 words or fewer. Because the abstract will be published separately by abstracting services, it must be complete and understandable without reference to the text. It should be written in an impersonal form. Abbreviations, diagrams and references are not allowed.


Five keywords or short phrases should be given below the abstract. If names of microorganisms are used, they should precede the key words, and be followed by the latter in alphabetical order. All keywords should be relevantly connected with the subject matter (avoid common terms like: bacteria, medium, soil, temperature etc.) as they will be used for indexing purposes.


The introduction should provide background information to allow the reader to understand and evaluate the results of the present study and describe the purpose of the undertaken research. However, broad “academic lectures” on the subject and extensive literature reviews should be avoided.

Materials and Methods

This section should contain description of materials (biological and others) used and sufficient technical information so that the experiments can be repeated. For commonly used materials and methods (e.g. commonly used media, protein determination) a simple reference is sufficient. Novel or modified procedures should be described in detail.
When a large number of microbial strains or mutants were used in a study, include strain tables identifying the sources and properties of the strains, mutants, bacteriophages, plasmids, etc.


In the Results section, only the results of the experiments should be include; reserve extensive interpretation of the results for the Discussion section. When justified by the nature of the paper the Results and Discussionsections may be combined into Results and Discussionchapter. The results should be presented as concisely as possible and illustrated with tables or figures if applicable (presentation of the same results in both tables and figures is not acceptable).


The Discussion should provide an interpretation of the obtained results in relation to previously published work and to the experimental system at hand and should not contain extensive repetition of the Resultssection or reiteration of the introduction. In some papers the Results and Discussion can be combined into one section as mentioned above.
Acknowledgements for financial support and for a personal assistance (with the permission of person named) are given in two separate paragraphs below the main text.

Literature (and references)

In text, references should be cited by the names of the authors and the year of publication, e.g. “Nowak and Kowalski (1999) stated that…”; “as previously described (Nowak and Kowalski, 2000; Nowak, 2005)”. When a paper has more than two authors, the first author’s name should be followed with et al. and the year of publication, e.g. Nowak et al., 2002. While references occur that are not identified by the authors’ names and year, use a, b etc. after the year (Nowak et al., 2002a; 2002b).

The list of the papers cited (Literature) must be arranged alphabetically according to the last name of the first author and not numbered. Papers with one only author are listed in chronological order (the earliest first); papers with two authors are listed alphabetically according to the last name of the first author and by the last name of the second author; paper with three or more authors appear as those with two authors and are listed chronologically. When the paper has more than ten authors, give the names of the first ten, followed by “and others”. For citations of books, books chapters, thesis, printed conference proceedings etc. see examples given below.

Please follow the punctuations, brackets, capital letters etc. exactly as shown. Put “and”, not “&” before the last author’s name. Use the proper journal title abbreviations (ISO) as indicated in the PubMed Journal Database.